Respiration in Plants ISC Class 11: Glycolysis, Krebs Cycle, ETS and RQ
Updated 15 October 2026 · Fact-checked
Respiration in Plants is a chapter in Unit 4 (Plant Physiology) of ISC Class 11 Biology. It covers gas exchange in plants, aerobic and anaerobic respiration, flowcharts of glycolysis, the Krebs cycle and the electron transport system, fermentation, oxidative phosphorylation, the ATP count per glucose, the amphibolic pathway and respiratory quotient values.
What Respiration in Plants is about
Every living cell needs a steady supply of energy. Cellular respiration releases the energy stored in food by breaking C–C bonds through oxidation, and traps part of it as ATP, the cell's energy currency. The molecules oxidised are respiratory substrates, usually carbohydrates, but also fats and proteins.
Plants have no special breathing organs. Each part exchanges gases through stomata and lenticels, and cells are close to the surface or to air spaces, so diffusion suffices.
Glycolysis in the cytoplasm splits glucose into two pyruvate molecules with a small gain of ATP and NADH. What happens next depends on oxygen:
- Without O₂ (anaerobic respiration / fermentation), pyruvate becomes ethanol + CO₂ (yeast) or lactic acid (some bacteria, muscle), giving only 2 ATP per glucose.
- With O₂ (aerobic respiration), pyruvate enters the mitochondrion, becomes acetyl CoA, and is fully oxidised to CO₂ in the Krebs (TCA) cycle. The NADH and FADH₂ formed pass electrons along the electron transport system to oxygen, and the energy released makes most of the ATP by oxidative phosphorylation.
In the classical count, one glucose gives 38 ATP aerobically. The respiratory pathway is amphibolic, used for both breakdown and synthesis. The respiratory quotient (RQ), CO₂ released ÷ O₂ used, tells us which substrate is being respired: about 1 for carbohydrates, 0.7 for fats and 0.9 for proteins.
The syllabus asks for flowcharts only of glycolysis, the Krebs cycle and ETS, a definition of oxidative phosphorylation and a brief idea of fermentation and the amphibolic pathway.
Weightage: Respiration in Plants belongs to the unit Plant Physiology, which CISCE weights at 17 of the 70 marks in the ISC Class XI Biology Paper I (Theory). Class XI is examined by the school, so your school sets the paper within this weightage.Source: CISCE ISC Biology syllabus, examination year 2028, Class XI unit weightage
Key concepts
- Gas exchange in plants and types of respiration
Do plants breathe? Plants need O₂ for respiration and give out CO₂, but they have no special respiratory organs. Reasons: (1) each part (root, stem, leaf) takes care of its own gas exchange, so little gas is transported from one part to another; (2) plants have low respiratory demand compared with animals, except during germination and active growth; (3) gases diffuse only short distances: almost every living cell is close to the surface, and loosely packed parenchyma has intercellular air spaces connected to stomata (leaves, young stems) and lenticels (woody stems and roots); in large stems most living cells lie just under the bark. In leaves, the O₂ produced in photosynthesis is also available to cells. Types of respiration: (1) aerobic, using O₂, completely oxidising the substrate to CO₂ and water and releasing much energy; (2) anaerobic, without O₂, incompletely oxidising the substrate to ethanol or lactic acid; in microbes it is called fermentation. Sometimes respiration is also classed by substrate: floating respiration uses carbohydrates or fats; protoplasmic respiration uses proteins (in starving cells).
Worked example
A large tree trunk has no breathing organ. How do its living cells get oxygen?
Try it first, then show the solutionSolution
- Most of the trunk's interior is dead wood that does not respire
- The living cells lie in a thin layer just under the bark and get O₂ by diffusion through lenticels and air spaces
Answer: Diffusion through lenticels to living cells near the surface
- Glycolysis (EMP pathway): flowchart
Glycolysis (Embden–Meyerhof–Parnas pathway) occurs in the cytoplasm of all living cells and needs no oxygen. In plants the glucose comes from sucrose or from stored starch. Flowchart: glucose (6C) → *(ATP → ADP; hexokinase)* glucose-6-phosphate → fructose-6-phosphate → *(ATP → ADP; phosphofructokinase)* fructose-1,6-bisphosphate (6C) → splits into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate (PGAL), two interconvertible 3C triose phosphates → each PGAL *(NAD⁺ → NADH + H⁺; inorganic phosphate added)* → 1,3-bisphosphoglycerate → *(ADP → ATP)* 3-phosphoglycerate → 2-phosphoglycerate → *(−H₂O)* phosphoenolpyruvate (PEP) → *(ADP → ATP)* pyruvic acid (3C). Balance per glucose: ATP used 2, ATP formed 4 (by substrate-level phosphorylation), so net 2 ATP; 2 NADH formed; 2 pyruvate formed. Pyruvic acid is the key product: its fate depends on whether O₂ is available.
Worked example
In glycolysis, at which two steps is ATP used and at which steps is it formed?
Try it first, then show the solutionSolution
- Used: glucose → glucose-6-phosphate, and fructose-6-phosphate → fructose-1,6-bisphosphate
- Formed: 1,3-bisphosphoglycerate → 3-phosphoglycerate, and PEP → pyruvate (each twice per glucose)
Answer: 2 ATP used; 4 ATP formed; net gain 2 ATP
- Fermentation (anaerobic respiration): brief idea
Under anaerobic conditions pyruvate is not oxidised further; instead the NADH from glycolysis is used to reduce it, regenerating NAD⁺ so that glycolysis can continue. (1) Alcoholic fermentation (yeast, also plant roots in waterlogged soil): pyruvic acid → *(pyruvic acid decarboxylase; CO₂ released)* acetaldehyde → *(alcohol dehydrogenase; NADH → NAD⁺)* ethanol. (2) Lactic acid fermentation (some bacteria; animal muscle during intense exercise when O₂ is short): pyruvic acid → *(lactate dehydrogenase; NADH → NAD⁺)* lactic acid. In both, the net yield is only 2 ATP per glucose (from glycolysis), and most of the energy remains in ethanol or lactic acid. These products are toxic; yeasts die when ethanol reaches about 13% in the medium.
Worked example
Why must yeast convert pyruvate to ethanol rather than simply stopping at pyruvate when O₂ is absent?
Try it first, then show the solutionSolution
- Glycolysis needs NAD⁺ at the PGAL oxidation step; without O₂ the NADH cannot be oxidised by the ETS
- Reducing acetaldehyde to ethanol oxidises NADH back to NAD⁺, letting glycolysis continue and make ATP
Answer: To regenerate NAD⁺ so that glycolysis keeps making ATP
- Aerobic respiration: link reaction and Krebs cycle (flowchart)
In the presence of O₂, pyruvate enters the mitochondrial matrix. Link reaction (oxidative decarboxylation): pyruvic acid (3C) + coenzyme A + NAD⁺ → *(pyruvate dehydrogenase complex; Mg²⁺)* acetyl CoA (2C) + CO₂ + NADH + H⁺. Krebs cycle (citric acid / TCA cycle, described by Hans Krebs) in the matrix: acetyl CoA (2C) + oxaloacetic acid (4C) + H₂O → *(citrate synthase; CoA released)* citric acid (6C) → isocitrate (6C) → *(NAD⁺ → NADH; CO₂ out)* α-ketoglutaric acid (5C) → *(NAD⁺ → NADH; CO₂ out)* succinyl CoA (4C) → *(GDP → GTP → ATP; substrate-level phosphorylation)* succinic acid (4C) → *(FAD → FADH₂)* fumaric acid → *(+H₂O)* malic acid → *(NAD⁺ → NADH)* oxaloacetic acid (4C), which accepts the next acetyl CoA. Per turn (per acetyl CoA): 2 CO₂, 3 NADH, 1 FADH₂, 1 ATP (via GTP). Each glucose gives two pyruvates, so the cycle turns twice. Up to the Krebs cycle the glucose has been fully broken to CO₂, but most energy is still held in NADH and FADH₂.
Worked example
How many CO₂, NADH and FADH₂ are produced from one pyruvate entering the mitochondrion (link reaction plus one Krebs turn)?
Try it first, then show the solutionSolution
- Link reaction: 1 CO₂ and 1 NADH
- Krebs turn: 2 CO₂, 3 NADH, 1 FADH₂ (and 1 ATP)
Answer: 3 CO₂, 4 NADH and 1 FADH₂ (plus 1 ATP)
- Electron transport system and oxidative phosphorylation
The electron transport system (ETS) lies in the inner mitochondrial membrane. Flowchart: NADH (from the matrix) → complex I (NADH dehydrogenase) → ubiquinone (coenzyme Q) → complex III (cytochrome bc₁) → cytochrome c (a small mobile protein on the outer face of the inner membrane) → complex IV (cytochrome c oxidase, with cytochromes a and a₃ and copper) → O₂, which accepts the electrons and protons to form water. FADH₂ enters through complex II (succinate dehydrogenase) to ubiquinone, by-passing complex I. As electrons flow, energy is used to pump protons from the matrix into the intermembrane space, building a proton gradient. Protons flow back through complex V (ATP synthase: an F₀ channel in the membrane and an F₁ head facing the matrix), which makes ATP. Oxidative phosphorylation (definition): the synthesis of ATP from ADP and inorganic phosphate using the energy released when electrons from NADH and FADH₂ are passed along the ETS to oxygen as the final acceptor. It differs from photophosphorylation, where light energy drives the proton gradient. Oxygen is needed only at the end of the chain, but without it the whole chain stops, because it removes the electrons.
Worked example
Why does cyanide, which blocks cytochrome c oxidase, stop almost all ATP production in a cell, even though glycolysis does not need oxygen?
Try it first, then show the solutionSolution
- With complex IV blocked, electrons cannot pass to O₂, so the whole chain backs up and no proton gradient is made
- NADH and FADH₂ cannot be reoxidised, so the Krebs cycle stops; only glycolysis (2 ATP per glucose) remains
Answer: Oxidative phosphorylation, which makes most ATP, stops completely
- Energy relations: ATP count per glucose
The classical balance sheet assumes each NADH yields 3 ATP and each FADH₂ yields 2 ATP in the ETS, and that NADH made in the cytoplasm enters the mitochondrion without loss. Glycolysis: 2 ATP (net) + 2 NADH (= 6 ATP) → 8 ATP. Link reaction (×2): 2 NADH → 6 ATP. Krebs cycle (×2): 2 ATP + 6 NADH (18 ATP) + 2 FADH₂ (4 ATP) → 24 ATP. Total = 8 + 6 + 24 = 38 ATP per glucose, of which 4 are made by substrate-level phosphorylation and 34 by oxidative phosphorylation. This is a theoretical maximum: it assumes a strictly sequential, orderly pathway with one substrate, no intermediates withdrawn for synthesis, and full efficiency. In living cells some intermediates are used to build other molecules, transporting cytoplasmic NADH into mitochondria can cost energy, and the membrane is somewhat leaky, so the real yield is lower (often given as about 30–32 ATP). In fermentation the yield is only 2 ATP per glucose. Taking roughly 7.3 kcal per mole of ATP and 686 kcal per mole of glucose, aerobic respiration traps about 40% of the energy as ATP; the rest is lost as heat.
Worked example
How many ATP are produced by the complete aerobic oxidation of 5 molecules of glucose, and how many glucose molecules would yeast need to ferment to produce the same amount?
Try it first, then show the solutionSolution
- Aerobic: 5 × 38 = 190 ATP
- Fermentation gives 2 ATP per glucose: 190 ÷ 2 = 95 glucose molecules
Answer: 190 ATP; 95 glucose molecules
- Amphibolic pathway: brief idea
The respiratory pathway is not only for breakdown (catabolism). Its intermediates are also used to build molecules (anabolism), so it is called amphibolic. Entry points for breakdown: carbohydrates enter as glucose; fats are broken into glycerol, which enters glycolysis as PGAL, and fatty acids, which are broken down to acetyl CoA; proteins are broken into amino acids, which after removal of the amino group enter as pyruvate, acetyl CoA or Krebs-cycle acids such as α-ketoglutarate and oxaloacetate. Withdrawals for synthesis: when a cell needs to make fatty acids, acetyl CoA is withdrawn from the pathway; Krebs-cycle acids such as α-ketoglutarate and oxaloacetate are used to make amino acids. Thus the same pathway can run materials towards breakdown or away for synthesis.
Worked example
Why is the respiratory pathway called amphibolic and not just catabolic?
Try it first, then show the solutionSolution
- Fats and proteins are broken down and fed into it, which is catabolic
- Acetyl CoA and Krebs acids are also withdrawn to make fatty acids and amino acids, which is anabolic
Answer: Because it serves both breakdown and synthesis
- Respiratory quotient (RQ)
The respiratory quotient is the ratio of the volume of CO₂ evolved to the volume of O₂ consumed in respiration: RQ = CO₂ ÷ O₂. It depends on the substrate. (1) Carbohydrates: , so RQ = 6/6 = 1. (2) Fats contain relatively little oxygen, so they need more O₂ to oxidise; for tripalmitin, , RQ = 102/145 = 0.7 (less than 1). (3) Proteins: about 0.9. (4) Organic acids, which are rich in oxygen, give RQ more than 1: malic acid, , RQ = 4/3 = 1.33. (5) Anaerobic respiration: CO₂ is released but no O₂ is used, so RQ is infinite. In living organisms the substrate is usually a mixture, so measured RQ values are intermediate; e.g. an RQ near 0.7 in germinating castor seeds shows that fat is being used.
Worked example
Germinating seeds use 14 mL of O₂ and give out 10 mL of CO₂ in an hour. Calculate the RQ and identify the likely substrate.
Try it first, then show the solutionSolution
- RQ = CO₂ ÷ O₂ = 10 ÷ 14 = 0.71
- An RQ near 0.7 indicates fats
Answer: RQ ≈ 0.71; the seeds are respiring fat (oily seeds)
Respiration in Plants in real life
Each concept, met in an everyday situation. Read the situation, answer the question in your own words, then compare with the model answer. The one-line hook is what to remember.
Making idli and bread
Suppose idli batter or bread dough is left overnight and swells up with bubbles.
Which process makes the bubbles, and what else is produced?
Try it first, then show the solutionSolution
- Yeast and bacteria respire the sugars anaerobically (fermentation)
- Alcoholic fermentation gives CO₂, which forms bubbles, and ethanol, which evaporates during cooking
Answer: Fermentation produces CO₂ that makes the dough rise.
Memory hook: Yeast puffs with CO₂.
Waterlogged fields
Imagine a maize field that stays flooded for several days after heavy rain, and the plants begin to wilt and yellow.
Why does waterlogging harm roots?
Try it first, then show the solutionSolution
- Water fills the soil air spaces, so roots get little O₂ and switch to anaerobic respiration
- Fermentation gives only 2 ATP per glucose, too little for ion uptake, and ethanol builds up to toxic levels
Answer: Roots run short of energy and are poisoned by ethanol, so water and mineral uptake fails.
Memory hook: No air, no ATP.
Cramps after a sprint
Suppose a student sprints 400 m and feels a burning sensation in the leg muscles.
Which type of respiration caused this?
Try it first, then show the solutionSolution
- O₂ supply to the muscles could not keep up with demand
- Muscle cells made extra ATP by glycolysis and reduced pyruvate to lactic acid, which built up
Answer: Lactic acid fermentation in muscle.
Memory hook: Lactic in the legs.
Topics in this chapter
Respiration in Plants: key facts
- Aerobic respiration of glucose
- About 686 kcal per mole released.
- Respiratory quotient
- Carbohydrate 1; fat about 0.7; protein about 0.9; organic acids above 1; anaerobic infinite.
- ATP balance per glucose (classical)
- NADH = 3 ATP, FADH₂ = 2 ATP; actual yield lower.
- Per pyruvate and per acetyl CoA
- Useful for quick checks.
- Fermentation
- NADH reoxidised; net 2 ATP per glucose.
Respiration in Plants: diagrams to practise
Practise each one on paper: draw it from the checklist, then tick off every label.
Flowchart of glycolysis
Glycolysis (only flowchart).
Draw
- A vertical chain: glucose → glucose-6-P → fructose-6-P → fructose-1,6-bisP.
- A split into DHAP ⇌ PGAL (glyceraldehyde-3-P).
- Below PGAL, marked ×2: 1,3-bisphosphoglycerate → 3-PGA → 2-PGA → PEP → pyruvic acid.
- Side arrows: ATP → ADP at two upper steps; NAD⁺ → NADH at PGAL step; ADP → ATP at two lower steps; H₂O out at 2-PGA → PEP.
Label
- glucose
- fructose-1,6-bisphosphate
- PGAL
- DHAP
- 1,3-bisphosphoglycerate
- PEP
- pyruvic acid
- ATP used
- ATP formed
- NADH
Watch out: Write 'net 2 ATP + 2 NADH + 2 pyruvate' in a box at the bottom.
Flowchart of the Krebs cycle
TCA cycle (only flowchart).
Draw
- Above the circle: pyruvic acid → acetyl CoA (CO₂ and NADH out).
- A circle: acetyl CoA + OAA → citric acid → isocitric acid → α-ketoglutaric acid → succinyl CoA → succinic acid → fumaric acid → malic acid → OAA.
- Side arrows: CO₂ out at two steps; NADH at three steps; GTP/ATP at succinyl CoA → succinate; FADH₂ at succinate → fumarate.
Label
- acetyl CoA
- citric acid (6C)
- α-ketoglutaric acid (5C)
- succinic acid (4C)
- oxaloacetic acid (4C)
- CO₂
- NADH
- FADH₂
- ATP (GTP)
Watch out: Write carbon numbers beside each acid; they show where CO₂ leaves.
Electron transport system
ETS (only flowchart) or oxidative phosphorylation.
Draw
- The inner mitochondrial membrane drawn horizontally: matrix below, intermembrane space above.
- Boxes I, III and IV in the membrane, with ubiquinone (Q) between I and III and cytochrome c between III and IV; complex II feeding Q from FADH₂.
- Electron arrows from NADH to I → Q → III → cyt c → IV → ½O₂ + 2H⁺ → H₂O.
- Proton arrows into the intermembrane space; complex V (ATP synthase) letting H⁺ back with ADP + Pi → ATP.
Label
- NADH
- complex I
- FADH₂
- complex II
- ubiquinone
- complex III
- cytochrome c
- complex IV
- O₂ → H₂O
- ATP synthase (F₀–F₁)
Watch out: Oxygen is the final electron acceptor; show water formed at complex IV.
Interrelationship of respiratory pathways (amphibolic)
Amphibolic pathway.
Draw
- Three boxes at the top: carbohydrates, fats, proteins.
- Arrows: carbohydrates → glucose → glycolysis; fats → glycerol → PGAL and fatty acids → acetyl CoA; proteins → amino acids → pyruvate, acetyl CoA, Krebs acids.
- Double-headed arrows at acetyl CoA and Krebs acids to show withdrawal for synthesis.
Label
- glucose
- glycerol
- fatty acids
- amino acids
- pyruvate
- acetyl CoA
- Krebs cycle
- breakdown
- synthesis
Watch out: The two-way arrows are what make it amphibolic.
How the school exam asks Respiration in Plants
The question patterns to expect, and the method that works for each.
| Question type | How it is asked | Method |
|---|---|---|
| Flowcharts | Give a flowchart of glycolysis, the Krebs cycle or the ETS. | Names of intermediates in order with ATP, NADH, FADH₂, CO₂ arrows. |
| Definitions and differences | Define oxidative phosphorylation; distinguish aerobic and anaerobic respiration; alcoholic and lactic fermentation. | Matched points: site, O₂ need, products, ATP yield. |
| ATP accounting | Calculate net ATP from one glucose; from a given number of glucose, pyruvate or acetyl CoA. | Stage-by-stage table; state the 3 and 2 conversion values. |
| RQ | Define RQ; calculate from data or equations; explain values for carbohydrates, fats, proteins and acids. | CO₂ ÷ O₂ with the balanced equation. |
| Reasoning | Why do plants lack respiratory organs? Why is the pathway amphibolic? Why does waterlogging harm roots? | Link structure, oxygen and energy. |
Tricks to remember Respiration in Plants
Short memory aids. Say each one aloud once, then use it on a question the same day.
Citrate Is Krebs' Starting Substrate For Making Oxaloacetate
Citric acid, Isocitric, α-Ketoglutaric, Succinyl CoA, Succinic, Fumaric, Malic, Oxaloacetic.
Use it when: Order of Krebs cycle acids.
2-2-6-2 for one glucose through Krebs
2 ATP, 2 FADH₂, 6 NADH, 4 CO₂ (two turns).
Use it when: Krebs cycle totals.
8 + 6 + 24 = 38
Glycolysis 8, link 6, Krebs 24.
Use it when: ATP count.
One, point-seven, point-nine
RQ: carbohydrate 1, fat 0.7, protein 0.9; acids above 1; anaerobic infinite.
Use it when: RQ values.
I-Q-III-c-IV-O
NADH → complex I → ubiquinone → complex III → cytochrome c → complex IV → oxygen.
Use it when: ETS order.
Respiration in Plants: exam-level solved questions
Ordered from exam level to HOTS. Cover the solution, write your own, then compare step by step.
Example 1: Site of glycolysis · 1 mark · MCQExam level
Glycolysis takes place in the:
(a) mitochondrial matrix
(b) inner mitochondrial membrane
(c) cytoplasm
(d) chloroplast
Try it first, then show the solutionSolution
- Glycolysis is common to aerobic and anaerobic respiration and happens in the cytoplasm.
Answer: (c)
Example 2: RQ of fats · 1 mark · Assertion-reasonHarder exam level
Assertion: The RQ of fats is less than one.
Reason: Fats are poor in oxygen relative to carbon and hydrogen, so more O₂ is consumed than CO₂ released.
(a) Both Assertion and Reason are true, and Reason is the correct explanation for Assertion.
(b) Both Assertion and Reason are true, but Reason is not the correct explanation for Assertion.
(c) Assertion is true and Reason is false.
(d) Assertion is false and Reason is true.
Try it first, then show the solutionSolution
- Tripalmitin: 102 CO₂ for 145 O₂ gives RQ 0.7; this is because of its low oxygen content.
Answer: (a)
Example 3: Oxidative phosphorylation · 2 marks · Short answerHarder exam level
Define oxidative phosphorylation. How does it differ from photophosphorylation? [2]
Try it first, then show the solutionSolution
- Synthesis of ATP using energy released when electrons from NADH and FADH₂ pass along the ETS to O₂ (final acceptor) in mitochondria
- In photophosphorylation light energy drives electron flow and ATP synthesis in chloroplasts; no O₂ is needed as acceptor
Answer: As above
Example 4: Fermentation types · 3 marks · Short answerHarder exam level
Distinguish between alcoholic and lactic acid fermentation with a flowchart of each. [3]
Try it first, then show the solutionSolution
- Alcoholic: pyruvic acid → acetaldehyde + CO₂ (pyruvic decarboxylase) → ethanol (alcohol dehydrogenase, NADH → NAD⁺); yeast
- Lactic: pyruvic acid → lactic acid (lactate dehydrogenase, NADH → NAD⁺); no CO₂; bacteria, muscle
- Both net 2 ATP per glucose
Answer: As above
Example 5: ATP balance sheet · 5 marks · Long answerHarder exam level
(a) Prepare a balance sheet of ATP formed during the complete aerobic oxidation of one glucose molecule. [3]
(b) Why is 38 ATP considered a theoretical value? [2]
Try it first, then show the solutionSolution
- (a) Glycolysis: 2 ATP + 2 NADH (6) = 8
- Link reaction: 2 NADH = 6
- Krebs: 2 ATP + 6 NADH (18) + 2 FADH₂ (4) = 24; total 38
- (b) It assumes a sequential pathway, glucose as the only substrate, no intermediates withdrawn and NADH from glycolysis entering mitochondria without cost
- In cells, intermediates are used for synthesis, shuttles cost energy and membranes leak protons, so yields are lower
Answer: 38 ATP (theoretical)
Example 6: RQ calculations · 3 marks · Short answerHarder exam level
Calculate the RQ for (i) glucose (ii) malic acid () (iii) tripalmitin (). Explain why the values differ. [3]
Try it first, then show the solutionSolution
- (i) 6/6 = 1
- (ii) 4/3 = 1.33; (iii) 102/145 = 0.70
- Oxygen-rich organic acids need less O₂ (RQ > 1); oxygen-poor fats need more O₂ (RQ < 1)
Answer: 1; 1.33; 0.70
Example 7: Counting from pyruvate · 3 marks · Short answerHOTS / competency
A mitochondrial preparation is supplied with 10 molecules of pyruvate and plenty of O₂. Using classical values, how many (a) CO₂ (b) NADH (c) ATP will be produced? [3]
Try it first, then show the solutionSolution
- Per pyruvate (link + one Krebs turn): 3 CO₂, 4 NADH, 1 FADH₂, 1 ATP
- (a) 30 CO₂ (b) 40 NADH (and 10 FADH₂)
- (c) ATP per pyruvate = 4 × 3 + 1 × 2 + 1 = 15; total 150 ATP
Answer: 30 CO₂; 40 NADH; 150 ATP
Example 8: Seeds and RQ · 3 marks · Short answerHOTS / competency
In an experiment, germinating wheat seeds show an RQ close to 1, while germinating castor seeds show an RQ near 0.7. Later in castor germination the RQ rises towards 1. Explain all three observations. [3]
Try it first, then show the solutionSolution
- Wheat seeds store starch: carbohydrate respiration gives RQ 1
- Castor seeds store oil: fats need more O₂ per CO₂, giving RQ about 0.7
- Later, stored fat is converted to sugars (and green seedlings make carbohydrate), so the substrate becomes carbohydrate and RQ approaches 1
Answer: Substrate shifts from fat to carbohydrate
Case-study practice (Section D style)
Read the situation, then answer every part. In a school exam set on the board pattern this is a 5-mark question split into 1 + 1 + 1 + 2 marks.
A yeast experiment
Suppose two flasks of sugar solution with yeast are set up: flask A is sealed and flask B is bubbled with air. Both are kept at 30 °C.
(i) · 1 mark
In which flask will ethanol be formed?
Answer
Answer: Flask A
No O₂: fermentation.
(ii) · 1 mark
Which flask's yeast will multiply faster? Why?
Answer
Answer: Flask B; aerobic respiration gives about 19 times more ATP per glucose
38 vs 2 ATP.
(iii) · 1 mark
What is the RQ in flask A?
Answer
Answer: Infinite (CO₂ released, no O₂ used)
Anaerobic.
(iv) · 2 marks
Why does fermentation in flask A eventually stop even if sugar remains?
Answer
Answer: Ethanol accumulates and becomes toxic to the yeast (around 13%)
Product inhibition.
A cyanide-poisoned tissue
Imagine a researcher treats a slice of potato tuber with a chemical that blocks complex IV of the ETS.
(i) · 1 mark
Name complex IV.
Answer
Answer: Cytochrome c oxidase
Passes electrons to O₂.
(ii) · 1 mark
What happens to O₂ uptake by the tissue?
Answer
Answer: It falls almost to zero
O₂ is the final acceptor at complex IV.
(iii) · 1 mark
Which stage of respiration can still make ATP?
Answer
Answer: Glycolysis (followed by fermentation)
Does not need the ETS.
(iv) · 2 marks
Explain why the Krebs cycle also stops.
Answer
Answer: NADH and FADH₂ cannot be reoxidised, so NAD⁺ and FAD run out and the Krebs cycle dehydrogenases cannot work
Coupling of Krebs cycle and ETS.
Where the marks are
How a typical answer is split into step marks. Show every step: a right final answer alone rarely earns full marks.
Why do plants not need specialised respiratory organs? Give two reasons. (2 marks)
| Step | Marks |
|---|---|
| Each part handles its own gas exchange, and respiratory demand is low except in growing regions | 1 |
| Living cells are close to the surface or to air spaces connected with stomata and lenticels, so diffusion is enough | 1 |
What is RQ? State its value for carbohydrates, fats and proteins. (3 marks)
| Step | Marks |
|---|---|
| RQ = volume of CO₂ evolved ÷ volume of O₂ consumed in respiration | 1 |
| Carbohydrates 1 (glucose: 6 CO₂/6 O₂) | 1 |
| Fats about 0.7; proteins about 0.9 | 1 |
Give a flowchart of the Krebs cycle and state the products of one turn. (5 marks)
| Step | Marks |
|---|---|
| Acetyl CoA + OAA → citric acid → isocitric acid | 1 |
| → α-ketoglutaric acid (CO₂, NADH) → succinyl CoA (CO₂, NADH) | 1 |
| → succinic acid (ATP via GTP) → fumaric acid (FADH₂) | 1 |
| → malic acid → oxaloacetic acid (NADH), regenerating the acceptor | 1 |
| Per turn: 2 CO₂, 3 NADH, 1 FADH₂, 1 ATP; site: mitochondrial matrix | 1 |
Where students lose marks in Respiration in Plants
The slips examiners see most often, and what to write instead.
Mistake: Counting glycolysis as 4 ATP
The investment steps are forgotten.
Wrong working
Glycolysis gives 4 ATP.
Right working
4 ATP are formed but 2 are used: net 2 ATP (plus 2 NADH).
Do this: Always write 'net'.
Usually costs: 1 mark.
Mistake: Counting the Krebs cycle once per glucose
One turn is learned, then not doubled.
Wrong working
Krebs gives 1 ATP, 3 NADH and 1 FADH₂ per glucose.
Right working
One glucose gives two acetyl CoA, so the cycle turns twice: 2 ATP, 6 NADH, 2 FADH₂.
Do this: Multiply post-glycolysis steps by two.
Usually costs: 1-2 marks.
Mistake: Inverting the RQ ratio
O₂ is written on top.
Wrong working
RQ of fat = 145/102 = 1.42.
Right working
RQ = CO₂/O₂ = 102/145 = 0.7.
Do this: Quotient of what comes out over what goes in: CO₂ over O₂.
Usually costs: 1-2 marks.
Mistake: Saying oxygen is used in the Krebs cycle
Krebs is linked to aerobic respiration.
Wrong working
O₂ combines with acetyl CoA in the Krebs cycle.
Right working
O₂ is used only at the end of the ETS as the final electron acceptor, forming water.
Do this: Oxygen waits at complex IV.
Usually costs: 1 mark.
Mistake: Writing full mechanisms instead of flowcharts
Over-preparation.
Wrong working
Every enzyme name and structural formula in glycolysis.
Right working
The syllabus asks for flowcharts only: intermediates in order with key inputs and outputs.
Do this: Practise a neat one-page flowchart for each.
Usually costs: Time.
Practice questions on Respiration in Plants
Write each answer on paper first. Open the hint only if you are stuck, and the answer only when you have finished.
Q1 · 1 mark · MCQExam level
The final acceptor of electrons in the ETS is:
(a) NAD⁺
(b) cytochrome c
(c) oxygen
(d) FADHint
Forms water.
Answer
(c)
Q2 · 1 mark · MCQExam level
The RQ of a carbohydrate is:
(a) 0.7
(b) 0.9
(c) 1
(d) infiniteHint
6/6.
Answer
(c)
Q3 · 1 mark · Very short answerExam level
Name the 6-carbon compound formed first in the Krebs cycle.
Hint
Citric acid cycle.
Answer
Citric acid
Q4 · 1 mark · Very short answerExam level
Name the end product of glycolysis.
Hint
3-carbon.
Answer
Pyruvic acid (pyruvate)
Q5 · 2 marks · Short answerExam level
Distinguish between aerobic and anaerobic respiration.
Hint
O₂, products, ATP.
Answer
Aerobic: uses O₂; complete oxidation to CO₂ and H₂O; in cytoplasm and mitochondria; 38 ATP per glucose. Anaerobic: no O₂; incomplete oxidation to ethanol + CO₂ or lactic acid; in cytoplasm only; 2 ATP per glucose.
Q6 · 2 marks · Short answerHarder exam level
What is the link reaction? Where does it occur?
Hint
Pyruvate to acetyl CoA.
Answer
Oxidative decarboxylation of pyruvic acid to acetyl CoA by the pyruvate dehydrogenase complex, releasing CO₂ and forming NADH; it occurs in the mitochondrial matrix and links glycolysis to the Krebs cycle.
Q7 · 2 marks · Short answerHarder exam level
A seedling is respiring stored fat. At which point of the respiratory pathway does (i) glycerol and (ii) a fatty acid enter? (iii) Name one Krebs-cycle acid that the same cell can withdraw to make amino acids.
Hint
Fats split into two parts that enter at different points.
Answer
(i) Glycerol is converted to PGAL (glyceraldehyde-3-phosphate) and enters glycolysis. (ii) Fatty acids are broken down to acetyl CoA, which enters the Krebs cycle. (iii) α-Ketoglutaric acid (or oxaloacetic acid). Because the pathway both receives breakdown products and supplies building blocks, it is amphibolic.
Q8 · 3 marks · Short answerHarder exam level
Distinguish between substrate-level phosphorylation and oxidative phosphorylation, naming one step or site where each occurs in aerobic respiration.
Hint
Is the phosphate moved directly from a substrate, or is a proton gradient used?
Answer
Substrate-level phosphorylation: ATP is formed by direct transfer of a phosphate group from a phosphorylated intermediate to ADP, without the ETS or O₂; e.g. PEP → pyruvic acid in glycolysis (cytoplasm) or succinyl CoA → succinic acid in the Krebs cycle (matrix). Oxidative phosphorylation: ATP is made by ATP synthase (F₀–F₁) in the inner mitochondrial membrane, using the proton gradient set up as electrons from NADH and FADH₂ pass along the ETS to O₂. Classically 4 of the 38 ATP per glucose come from the first and 34 from the second.
Q9 · 3 marks · Short answerHarder exam level
How many ATP are formed from (i) one acetyl CoA (ii) 4 molecules of glucose aerobically (iii) 4 molecules of glucose by fermentation?
Hint
12, 38, 2.
Answer
(i) 3 NADH × 3 + 1 FADH₂ × 2 + 1 ATP = 12 ATP (ii) 4 × 38 = 152 ATP (iii) 4 × 2 = 8 ATP.
Q10 · 2 marks · Short answerHarder exam level
A plant tissue consumed 25 mL of O₂ and released 20 mL of CO₂. Calculate its RQ and suggest the substrate.
Hint
CO₂ ÷ O₂.
Answer
RQ = 20 ÷ 25 = 0.8, between the values for fat (0.7) and protein (0.9): probably proteins or a mixture of fat and carbohydrate.
Q11 · 5 marks · Long answerHarder exam level
(a) Give a flowchart of glycolysis. [3]
(b) What is the net gain of ATP and NADH per glucose? Where is ATP formed by substrate-level phosphorylation? [2]Hint
Ten steps.
Answer
(a) Glucose → glucose-6-P (ATP used) → fructose-6-P → fructose-1,6-bisP (ATP used) → DHAP + PGAL → (×2) 1,3-bisphosphoglycerate (NADH formed) → 3-PGA (ATP formed) → 2-PGA → PEP → pyruvic acid (ATP formed). (b) Net 2 ATP and 2 NADH; substrate-level phosphorylation at 1,3-bisphosphoglycerate → 3-PGA and PEP → pyruvate.
Q12 · 5 marks · Long answerHarder exam level
(a) Describe the electron transport system and explain how ATP is made. [3]
(b) Why is oxygen essential although it is used only at the last step? [2]Hint
Final acceptor.
Answer
(a) In the inner mitochondrial membrane, electrons from NADH pass through complex I, ubiquinone, complex III, cytochrome c and complex IV to O₂; FADH₂ enters at complex II. Energy released pumps protons into the intermembrane space; protons return through ATP synthase (F₀–F₁), which makes ATP (oxidative phosphorylation). (b) O₂ removes electrons from the end of the chain; without it carriers stay reduced, electron flow and proton pumping stop, NADH cannot be reoxidised and the Krebs cycle also halts.
Q13 · 3 marks · Short answerHarder exam level
Why is the efficiency of aerobic respiration much higher than that of fermentation? Use approximate energy values (7.3 kcal per mole ATP; 686 kcal per mole glucose).
Hint
38 vs 2.
Answer
Aerobic: 38 × 7.3 = 277 kcal trapped out of 686, about 40%. Fermentation: 2 × 7.3 = 14.6 kcal, about 2%; most of the energy stays in ethanol or lactic acid because the substrate is only partly oxidised.
Q14 · 3 marks · Short answerHOTS / competency
During a long fast, a plant tissue shows an RQ of about 0.9 instead of 1. What does this suggest, and what kind of respiration is this?
Hint
Substrate change.
Answer
An RQ near 0.9 shows proteins are being used as the respiratory substrate after carbohydrate reserves are exhausted. Breakdown of the cell's own proteins is protoplasmic respiration; amino acids are deaminated and enter as pyruvate, acetyl CoA or Krebs acids (amphibolic pathway).
Q15 · 3 marks · Short answerHOTS / competency
A student says, 'In the Krebs cycle all the energy of glucose is released, since all its carbon leaves as CO₂.' Evaluate this statement.
Hint
Where is the energy?
Answer
Partly correct: by the end of the Krebs cycle all six carbons have left as CO₂. But only 4 ATP have been made directly; most energy is held as high-energy electrons in 10 NADH and 2 FADH₂. It is released only when these are oxidised in the ETS, giving 34 more ATP by oxidative phosphorylation.
Test your recall
Answer in your head or on paper first, then open the card. Pulling an answer out of memory makes it stick far better than re-reading.
1.Net ATP of glycolysis?Show answer
2 (plus 2 NADH).
2.Product of alcoholic fermentation?Show answer
Ethanol and CO₂.
3.Enzyme reducing pyruvate in muscle?Show answer
Lactate dehydrogenase.
4.Acceptor of acetyl CoA in Krebs cycle?Show answer
Oxaloacetic acid.
5.Site of ETS?Show answer
Inner mitochondrial membrane.
6.Complex IV?Show answer
Cytochrome c oxidase.
7.ATP per glucose (classical)?Show answer
38.
8.RQ in anaerobic respiration?Show answer
Infinite.
Best order to study it
- Learn gas exchange in plants and the types of respiration.
- Learn the glycolysis flowchart with ATP and NADH steps.
- Learn fermentation (alcoholic and lactic) in brief.
- Learn the link reaction and the Krebs cycle flowchart.
- Learn the ETS flowchart, oxidative phosphorylation and the ATP balance sheet.
- Finish with the amphibolic pathway and RQ calculations.
Quick revision checklist
The night before the exam: can you do each of these without looking?
- Can you give two reasons why plants lack respiratory organs?
- Can you draw the glycolysis flowchart?
- Can you write both fermentation pathways?
- Can you list the products of one Krebs turn?
- Can you name the ETS complexes in order?
- Can you define oxidative phosphorylation?
- Can you build the 38 ATP balance sheet?
- Can you calculate RQ for glucose, malic acid and tripalmitin?
Respiration in Plants: frequently asked questions
Should I write 38 or 36 ATP?
Use the classical 38 ATP per glucose (NADH = 3, FADH₂ = 2) unless your teacher says otherwise, and add that the actual yield in cells is lower because of shuttle costs, proton leakage and use of intermediates for synthesis.
Do I need enzyme names for glycolysis and the Krebs cycle?
The ISC 2028 syllabus asks for flowcharts only. Name the intermediates in order and show where ATP, NADH, FADH₂ and CO₂ are involved; a few key enzymes help but are not the focus.
Why is glycolysis thought to be an ancient pathway?
It occurs in the cytoplasm of almost all organisms, needs no oxygen and no membranes, which fits the early, oxygen-poor Earth.
Why is FADH₂ worth fewer ATP than NADH?
FADH₂ passes its electrons to ubiquinone through complex II, by-passing complex I, so fewer protons are pumped per pair of electrons.
Can RQ be more than 1?
Yes. When oxygen-rich organic acids such as malic or oxalic acid are respired, less O₂ is needed than the CO₂ released, so RQ exceeds 1.
Is fermentation the same as anaerobic respiration?
Fermentation is the name used for anaerobic breakdown of sugars by microbes such as yeast and bacteria. Higher organisms such as muscle and waterlogged roots carry out similar anaerobic respiration.
Based on: CISCE ISC 2028 Biology syllabus (Classes XI and XII); CISCE Regulations for the ISC Examination Year 2028 (Class XI internal examination)