CBSE Class 12 Biology 2026: question 20(a)
Set in the CBSE Class 12 board exam of 2026, paper 57/5/1, section B, question 20(a), for 2 marks. Try it first; the hints and our step-by-step solution are below.
Q20(a) · 2 marks · Very short answerBiotechnology and its Applications
Suggest how a virus-free healthy plant can be obtained from a diseased sugarcane plant.
Source: official question paper from cbse.gov.in (PDF). See it in the full 2026 board paper, set 57/5/1.
Hints
Open one hint at a time and try again before the next.
Hint 1
Even in an infected plant, one tissue usually remains free of virus.
Hint 2
Use the virus-free tip region as an explant in a technique that grows whole plants from small pieces of tissue.
Step-by-step solution
Each step shows the marks it earns, as an examiner would award them. Checked by an independent re-solve from the question paper.
- 1 mark
Remove the meristem (apical or axillary) from the diseased sugarcane plant. The meristem stays free of virus even when the rest of the plant is infected.
- 1 mark
Grow this meristem in vitro by tissue culture on a suitable nutrient medium. The plantlets that regenerate are virus-free healthy plants.
Answer: Meristem culture: excise the virus-free apical/axillary meristem and grow it by tissue culture to get virus-free plants.
Where the marks are
- States that the meristem is taken from the diseased plant because it is virus-free (1 mark)
- States that it is grown in vitro by tissue culture to obtain virus-free plants (1 mark)
Revise the chapter
- Chapter: Biotechnology and its Applications (notes, formulas, where marks are lost and every past question)
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The question is transcribed from the official paper; figures are described in words. The solution, hints and chapter tag are ours. Spotted an error? Write tosupport@questionbuddy.in.