CBSE Class 12 Biology 2026: question 23
Set in the CBSE Class 12 board exam of 2026, paper 57/5/1, section C, question 23, for 3 marks. Try it first; the hints and our step-by-step solution are below.
Q23 · 3 marks · Short answerMolecular Basis of Inheritance
Draw a labelled diagram of a 'replicating fork' showing the polarity. Why does DNA replication occur within such 'fork' ?
Source: official question paper from cbse.gov.in (PDF). See it in the full 2026 board paper, set 57/5/1.
Hints
Open one hint at a time and try again before the next.
Hint 1
DNA polymerase works only in the 5'→3' direction. Decide which new strand can be made continuously and which must be made in pieces.
Hint 2
For the reason, think about how long DNA is and what it costs to break all the hydrogen bonds at once.
Step-by-step solution
Each step shows the marks it earns, as an examiner would award them. Checked by an independent re-solve from the question paper.
- 2 marks
Diagram (described): Draw a Y-shaped replication fork. The parental double helix (one strand 5'→3', the other 3'→5') opens at the fork point (helicase site).
- On the template with 3'→5' polarity, the new leading strand is drawn as one continuous arrow, synthesised 5'→3' towards the fork.
- On the template with 5'→3' polarity, the new lagging strand is drawn as short separate pieces (Okazaki fragments), each synthesised 5'→3' away from the fork and joined by DNA ligase.
- Label the 5' and 3' ends of all four strands, mark DNA polymerase, and show the direction of replication with arrows.
- 1 mark
DNA is a very long molecule. Separating its two strands along the entire length at once would need a very high amount of energy. Replication therefore occurs within a small opening of the helix, the replication fork, where the strands unwind only a little at a time.
Answer: Labelled fork showing 3'→5' and 5'→3' templates, continuous leading strand, discontinuous lagging strand (Okazaki fragments). A fork is used because separating the full length of the long DNA molecule at once needs very high energy.
Where the marks are
- Correct fork diagram showing template polarity, continuous leading strand and discontinuous lagging strand with labels (2 marks)
- Reason: unwinding the full length of the long DNA needs too much energy, so replication proceeds in a small fork (1 mark)
Revise the chapter
- Chapter: Molecular Basis of Inheritance (notes, formulas, where marks are lost and every past question)
- Topic: DNA Replication
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