CBSE Class 12 Biology 2023: question 19(a)
Set in the CBSE Class 12 board exam of 2023, paper 57/1/1, section B, question 19(a), for 2 marks. Try it first; the hints and our step-by-step solution are below.
Q19(a) · 2 marks · Very short answerCompetency-basedBiotechnology: Principles and Processes
(i) Identify and name the structures 'A' and 'B' marked in the image given below :
(ii) State their importance in various biotechnology experiments.
Figure in the paper: (A) a circular double-stranded DNA (plasmid); (B) a bacteriophage with polyhedral head containing DNA, tail sheath and tail fibres. See it in the official paper.
Source: official question paper from cbse.gov.in (PDF). See it in the full 2023 board paper, set 57/1/1.
Hints
Open one hint at a time and try again before the next.
Hint 1
One structure is a small ring of DNA; the other is a virus with a head and tail.
Hint 2
What do we call the vehicles that carry a gene of interest into a host and multiply it?
Step-by-step solution
Each step shows the marks it earns, as an examiner would award them. Checked by an independent re-solve from the question paper.
- ½ mark
(i) 'A' is a plasmid, an extrachromosomal, circular, double-stranded DNA found in bacteria.
- ½ mark
'B' is a bacteriophage, a virus that infects bacteria.
- ½ mark
(ii) Plasmids are used as cloning vectors. They carry a foreign gene into the host cell and replicate independently of chromosomal DNA, often in high copy number. They can also carry selectable markers such as antibiotic-resistance genes.
- ½ mark
Bacteriophages are also used as cloning vectors. They can replicate inside bacterial cells independently of the chromosomal DNA and reach a high copy number per cell, so the linked foreign DNA is multiplied.
Answer: A – plasmid; B – bacteriophage; both act as cloning vectors that carry and multiply foreign DNA in a host
Where the marks are
- Identifies A as plasmid (½ mark)
- Identifies B as bacteriophage (½ mark)
- Importance of plasmid as vector (½ mark)
- Importance of bacteriophage as vector (½ mark)
Revise the chapter
- Chapter: Biotechnology: Principles and Processes (notes, formulas, where marks are lost and every past question)
- Topic: Cloning Vectors and pBR322
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The question is transcribed from the official paper; figures are described in words. The solution, hints and chapter tag are ours. Spotted an error? Write tosupport@questionbuddy.in.